Journal
/
Genetics
/
Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

DOI:
10.3791/59988-v

•

09:12 min

•

December 20, 2019

•

, , , , , ,
1Laboratory of Biology, BIO@SNS,Scuola Normale Superiore

Chapters

  • 00:05Title
  • 00:32Cell Transfection
  • 01:46Western Blot Sample Extraction
  • 05:00Western Blot
  • 06:51Results: Representative Tau Subcellular Enrichment Analyses
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

Tau is a neuronal protein present both in the cytoplasm, where it binds microtubules, and in the nucleus, where it exerts unconventional functions including the modulation of Alzheimer's disease-related genes. Here, we describe a method to investigate the function of nuclear Tau while excluding any interferences coming from cytoplasmic Tau.

Tags

TauSubcellular LocalizationNuclear FunctionSH-SY5YTransfectionTau-NLSTau-NESWestern BlotProtein ExtractionLysis Buffer

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Controllable Ion Channel Expression through Inducible Transient Transfection

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Read Article