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Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
DOI:

07:01 min

March 01, 2020

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Chapters

  • 00:05Introduction
  • 00:35Sphere Formation and Derived Adherent Population Generation
  • 02:28Sphere-forming Capacity Determination
  • 03:02Self-renewal Determination
  • 03:59Sphere Projection Area Determination
  • 04:51Results: Representative Endometrial and Breast Cancer Cell Sphere and Derived Adherent Population Analyses
  • 06:29Conclusion

Summary

Automatic Translation

The aim of this methodology is to identify cancer stem cells (CSC) in cancer cell lines and primary human tumor samples with the sphere-forming protocol, in a robust manner, using functional assays and phenotypic characterization with flow cytometry and Western blot.

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