Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Name: Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
Uploaded: 2020-03-01T12:00:00+00:00
Duration: 7 min 1 s
Description: Watch this Scientific Journal Video about Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors at JoVE.com

Mafalda Laranjo; Maria Jo&#227;o Carvalho; Beatriz Serambeque; Andr&#233; Alves; Carlos Miguel Marto; Isabel Silva; Artur Paiva; Maria Filomena Botelho

doi:10.3791/60022

Journal

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Cancer Research

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Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

DOI:

10.3791/60022-v

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07:01 min

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March 01, 2020

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Mafalda Laranjo*^1,2,3,4, Maria João Carvalho*^1,2,3,4,5,6, Beatriz Serambeque^2,3, André Alves⁷, Carlos Miguel Marto^2,3,4,8, Isabel Silva, Artur Paiva^9,10, Maria Filomena Botelho^2,3,4,8

¹Institute of Biophysics, Faculty of Medicine,University of Coimbra, ²Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine,University of Coimbra, ³CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, ⁴Clinical Academic Center of Coimbra (CACC), ⁵Universitary Clinic of Gynecology, Faculty of Medicine,University of Coimbra, ⁶Gynecology A Service,Coimbra Hospital and Universitary Center, ⁷Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine,University of Coimbra, ⁸Institute of Experimental Pathology, Faculty of Medicine,University of Coimbra, ⁹Cytometry Operational Management Unit, Clinical Pathology Service,Coimbra Hospital and Universitary Center, ¹⁰Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School,Laboratory Biomedical Sciences

Chapters

00:05Introduction
00:35Sphere Formation and Derived Adherent Population Generation
02:28Sphere-forming Capacity Determination
03:02Self-renewal Determination
03:59Sphere Projection Area Determination
04:51Results: Representative Endometrial and Breast Cancer Cell Sphere and Derived Adherent Population Analyses
06:29Conclusion

Summary

Automatic Translation

The aim of this methodology is to identify cancer stem cells (CSC) in cancer cell lines and primary human tumor samples with the sphere-forming protocol, in a robust manner, using functional assays and phenotypic characterization with flow cytometry and Western blot.