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Chronic Salmonella Infection Induced Intestinal Fibrosis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Chronic Salmonella Infection Induced Intestinal Fibrosis

Chronic Salmonella Infection Induced Intestinal Fibrosis

DOI:
10.3791/60068-v

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08:40 min

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September 22, 2019

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, , ,
1The Biomedical Research Centre,University of British Columbia

Chapters

  • 00:04Title
  • 01:30Preparation of Salmonella Typhimurium △AroA Cultures for Oral Gavage of Mice
  • 03:16Assessment of Salmonella Burdens in Tissues
  • 04:36Picrosirius Red Staining and Quantification of Collagen
  • 05:43Results: Chronic Salmonella Infection Induced Intestinal Fibrosis
  • 06:26Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a mouse model of Salmonella driven intestinal fibrosis that resembles key pathological hallmarks of Crohn’s disease including transmural inflammation and fibrosis. This method can be used to evaluate host factors that alter fibrotic outcomes using mutant mice maintained on a C57Bl/6 genetic background.

Tags

Intestinal FibrosisChronic InflammationCrohn’s DiseaseSalmonella InfectionMouse ModelOral GavageStreptomycinLB AgarLB BrothBacterial CultureSterile Technique

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