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Journal / Biology / CryoAPEX-metoden til elektronmikroskopianalyse af …
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CryoAPEX-metoden til elektronmikroskopianalyse af membranproteinlokalisering i ultrastrukturelt bevarede celler
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CryoAPEX-metoden til elektronmikroskopianalyse af membranproteinlokalisering i ultrastrukturelt bevarede celler

Article DOI: 10.3791/60677-v • 11:45 min • February 27th, 2020
February 27th, 2020 •
1, 2, 2,3,4, 1,4,5,6
1Department of Biological Sciences, Purdue University, 2Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 3Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, 4Bindley Biosciences Center, Purdue University, 5Purdue Institute of Integrative Neuroscience, Purdue University, 6Purdue Center for Cancer Research, Purdue University

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Denne protokol beskriver cryoAPEX-metoden, hvor et APEX2-mærket membranprotein kan lokaliseres ved transmissionselektronmikroskopi i optimalt bevaret celleultrastruktur.

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Biologi CryoAPEX membranprotein APEX2 transmissionelektronmikroskopi cryofixation højtryksfrysning frysesubstitution
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