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超结构保存细胞内膜蛋白定位电子显微镜分析的CryoAPEX方法
JoVE Journal
Biology
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JoVE Journal Biology
The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
DOI:

11:45 min

February 27, 2020

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Chapters

  • 00:05Title
  • 01:36Transfection, Chemical Fixation and Peroxidase Reaction
  • 03:48High Pressure Freezing
  • 04:46Freeze Substitution
  • 06:23Resin Infiltration and Embedding
  • 08:11Sectioning and TEM Imaging
  • 09:17Results: TEM Images
  • 11:07Conclusion

Summary

Automatic Translation

该协议描述了CryoAPEX方法,其中APEX2标记膜蛋白可以通过传输电子显微镜在最佳保存的细胞超结构内进行局部化。

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