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Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
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JoVE Journal Chemistry
Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

DOI:
10.3791/60690-v

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05:35 min

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January 17, 2020

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, , ,
1Department of Chemistry and Biochemistry,James Madison University, 2Biophysics Graduate Program,Ohio State University

Chapters

  • 00:04Title
  • 00:55Assay Component Preparation
  • 01:36Sample Preparation, Measurement, and Metal Quantification
  • 03:24Results: Absorbance Spectra and Ni2+ Quantification
  • 04:42Conclusion

Summary

Automatic Translation

Automatic Translation

We present an assay for easy quantification of metals introduced to samples prepared using immobilized metal affinity chromatography. The method uses hydroxynaphthol blue as the colorimetric metal indicator and a UV-Vis spectrophotometer as the detector.

Tags

Metal LeachingImmobilized Metal Affinity ChromatographyUV-Vis SpectrophotometryHNB ReagentProtein QuantificationBuffer SolutionSpectral AnalysisSample PreparationMetal Concentration Determination

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