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Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
DOI:

05:35 min

January 17, 2020

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Chapters

  • 00:04Title
  • 00:55Assay Component Preparation
  • 01:36Sample Preparation, Measurement, and Metal Quantification
  • 03:24Results: Absorbance Spectra and Ni2+ Quantification
  • 04:42Conclusion

Summary

Automatic Translation

We present an assay for easy quantification of metals introduced to samples prepared using immobilized metal affinity chromatography. The method uses hydroxynaphthol blue as the colorimetric metal indicator and a UV-Vis spectrophotometer as the detector.

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