A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels

Maribel Vazquez; Charity A. Dunn; Kenneth B. Walsh

doi:10.3791/3850

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Biology

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A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels

一个荧光识别GIRK通道调制器的筛选试验

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05:31 min

April 24, 2012

DOI: 10.3791/3850-v

Maribel Vazquez¹, Charity A. Dunn¹, Kenneth B. Walsh¹

¹Department of Pharmacology, Physiology & Neuroscience,University of South Carolina, School of Medicine

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一个确定药物相互作用与G蛋白门控内向整流钾实时甄别

该程序的总体目标是确定调节 grk 通道的新药。这是通过首先在 96 孔板中铺板 A TT 20 细胞来实现的。第二步是使用 Verset 液体板处理系统稀释测试化合物。

接下来，使用 verset 将稀释的测试化合物施加到细胞上。最后一步是将 96 孔板插入 Synergy 两个荧光读板器中，并将对照和生长抑素溶液注入孔中。最终，荧光读板器用于显示改变生长抑素诱导的荧光信号，从而调节 Grk 通道的化合物。

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