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JoVE Journal Bioengineering
Utvikling av en Flercellede Tredimensjonal organotypiske Model of Human tarmslimhinnen Grown Under Microgravity
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Utvikling av en Flercellede Tredimensjonal organotypiske Model of Human tarmslimhinnen Grown Under Microgravity

Article DOI: 10.3791/54148-v • 08:54 min • July 25th, 2016
July 25th, 2016 •
1, 2, 1
1Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, 2Mucosal Immunology and Biology Research Center, Massachusetts General Hospital for Children

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Celler som vokser i en tredimensjonal (3-D) miljø representerer en markert forbedring i forhold til celledyrking i 2-D-miljøer (f.eks flasker eller retter). Her beskriver vi utviklingen av en flercellet 3-D organotypiske modell av det menneskelige tarmslimhinnen dyrket under mikrogravitasjons tilgjengelig ved å dreie-vegg-fartøy (RWV) bioreaktorer.

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Bioengineering Human tredimensjonal (3-D) vektløshet roterende vegg kar (RWV) tarm epitelceller
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