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Journal / Chemistry / 液相色谱离子迁移 QTOF 质谱测定蛋白质生物中宿主细胞蛋白的 HS-MRM…
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液相色谱离子迁移 QTOF 质谱测定蛋白质生物中宿主细胞蛋白的 HS-MRM 法
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液相色谱离子迁移 QTOF 质谱测定蛋白质生物中宿主细胞蛋白的 HS-MRM 法

Article DOI: 10.3791/55325-v • 11:09 min • April 17th, 2018
April 17th, 2018 •
*1, *1, *1, 1, 1, 1
1Waters Corporation
* These authors contributed equally

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在这里, 我们描述了一种色谱检测, 再加上多肽前体离子迁移分离的高分辨率 (~ 3万) MS-肽片段的检测, 用于定量测定单克隆抗体中的尖肽标准消化。

Tags

化学 问题 134 宿主细胞蛋白 (HCPs) 超高性能液相色谱 (UPLC) 四极飞行时间质谱 (QTOF) 离子迁移光谱 (IMS) 多反应监测 (MRM) 选定的反应监测 (SRM) 高分辨率质谱 (HRMS) 定量 单克隆抗体 质谱 (MS) 液相色谱 (LC) 飞行后 MRM
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