二维和三维胚体小鼠胚胎干细胞的维甲酸诱导神经分化的分析
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09:04 min
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April 22, 2017
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我们描述了使用小鼠胚胎干细胞生成二维或三维的胚状体的技术。然后，我们解释如何以诱导胚状体细胞视黄酸，以及如何的神经分化由祖细胞标志物的免疫荧光和免疫印迹来分析其分化状态。
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Cite this Article
Yang, J., Wu, C., Stefanescu, I., Horowitz, A. Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies. J. Vis. Exp. (122), e55621, doi:10.3791/55621 (2017).
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