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Visualisering af endogene Mitophagy komplekser in situ i humane bugspytkirtel beta celler udnytte nærhed ligation assay

Article DOI: 10.3791/59398-v • 08:40 min • May 2nd, 2019
May 2nd, 2019 •
1, 1,2
1Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Michigan, Ann Arbor, 2VA Ann Arbor Healthcare System

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Denne protokol skitserer en metode til kvantitativ analyse af mitophagy protein kompleksdannelse specifikt i beta celler fra primære menneskelige ø prøver. Denne teknik giver således mulighed for analyse af mitophagy fra begrænset biologisk materiale, som er afgørende i dyrebare menneskelige bugspytkirtel beta celleprøver.

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Medicin bugspytkirtel beta celler mitophagy nærhed ligering assay humane Holme protein-interaktion mitokondrier
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