JoVE Journal
Developmental Biology
Developmental Biology
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
The blood-brain barrier (BBB) is a multicellular neurovascular unit tightly regulating brain homeostasis. By combining human iPSCs and organ-on-chip technologies, we have generated a personalized BBB chip, suitable for disease modeling and CNS drug penetrability predictions. A detailed protocol is described for the generation and operation of the BBB chip.