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Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

DOI:
10.3791/62326-v

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07:32 min

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February 17, 2021

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, , ,
1Centre for Atherothrombosis and Metabolic Disease, Hull York Medical School,University of Hull

Chapters

  • 00:05Introduction
  • 00:39Preparation of Skin for Wounding and Creating Ex Vivo Human Skin Wounds
  • 02:36Whole Mount Staining of Ex Vivo Wounds
  • 04:14Explant Imaging
  • 05:18Results: Wound Closure Evaluation by Whole-Mount Tissue Staining
  • 07:06Conclusion

Summary

Automatic Translation

Automatic Translation

Here we demonstrate an optimized technique for assessing wound repair using ex vivo human skin combined with a whole-mount staining approach. This methodology provides a pre-clinical platform for the evaluation of potential wound therapies.

Tags

Keywords: Ex VivoHuman SkinWound ModelWhole-mount StainingSkin RepairWound AssessmentSkin ExplantWound HealingSkin FixativeFluorescent Staining

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