Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Un ensayo celular basado en reporteros para…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biochemistry
Un ensayo celular basado en reporteros para monitorear la eficiencia del empalme
Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins
Journal (Biochemistry)
Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Journal (Biochemistry)
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
 
Click here for the English version

Un ensayo celular basado en reporteros para monitorear la eficiencia del empalme

Article DOI: 10.3791/63014-v • 08:53 min • September 15th, 2021
September 15th, 2021 •
1, 1, 1
1Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Este protocolo describe un ensayo de reportero de minigenes para monitorear el impacto de las mutaciones del sitio de empalme 5'en el empalme y desarrolla snRNA supresor U1 para el rescate de la inhibición del empalme inducida por la mutación. Las construcciones de snRNA U1 reportero y supresor se expresan en células HeLa, y el empalme se analiza por extensión de cebador o RT-PCR.

Tags

Bioquímica Número 175 Interacción ARN-proteína empalme pre-ARNm espliceosoma U1 snRNP intrón exón sitio de empalme reportero Dup51p salto de exón
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter