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Biochemistry

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JoVE Journal Biochemistry
Colunas de cromatografia de afinidade de membrana celular para identificar metabólitos de plantas especializadas interagindo com tropomyosina quinase imobilizada receptor B
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Colunas de cromatografia de afinidade de membrana celular para identificar metabólitos de plantas especializadas interagindo com tropomyosina quinase imobilizada receptor B

Article DOI: 10.3791/63118-v • 11:44 min • January 19th, 2022
January 19th, 2022 •
1,2, 1, 1, 1, 1
1Department of Biological Sciences, The University of Alabama, 2Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University

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O protocolo descreve a preparação de colunas de cromatografia de afinidade de membrana celular (CMAC) com fragmentos de membrana celular imobilizada contendo proteínas funcionais do receptor B transmembrano. Também é explicado o uso de colunas CMAC na identificação de metabólitos de plantas especializadas interagindo com esses receptores e presentes em complexas misturas naturais.

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Bioquímica Edição 179
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