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Bioengineering

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JoVE Journal Bioengineering
In vivo Imagem de tecidos biológicos com fluorescência combinada de dois fótons e microscopia de dispersão de Raman estimulada
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In vivo Imagem de tecidos biológicos com fluorescência combinada de dois fótons e microscopia de dispersão de Raman estimulada

Article DOI: 10.3791/63411-v • 09:06 min • December 20th, 2021
December 20th, 2021 •
1, 1, 1,2,3,4, 5
1Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center, The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences, Southern University of Science and Technology

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A microscopia estimulada de dispersão de Raman (SRS) permite imagens livres de rótulos de biomoléculas com base em sua vibração intrínseca de ligações químicas específicas. Neste protocolo, a configuração instrumental de um microscópio de srs e fluorescência de dois fótons integrado é descrita para visualizar estruturas celulares na medula espinhal de camundongos vivos.

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Bioengenharia Edição 178
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