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Et fluorescensbaseret assay af membranpotentiale til funktionel undersøgelse med høj kapacitet af to endogene ionkanaler i to epitelcellelinjer
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Et fluorescensbaseret assay af membranpotentiale til funktionel undersøgelse med høj kapacitet af to endogene ionkanaler i to epitelcellelinjer

Article DOI: 10.3791/63528-v • 06:59 min • June 22nd, 2022
June 22nd, 2022 •
1,3, 3, 2,4,5, 1,3,5
1Molecular Medicine, Hospital for Sick Children, 2Cell Biology, Hospital for Sick Children, 3Department of Physiology, University of Toronto, 4Department of Paediatrics, University of Toronto, 5Department of Biochemistry, University of Toronto

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Denne protokol beskriver en metode til undersøgelse af elektrogene membranproteiner ved at måle ændringer i membranpotentiale. Dette assay giver en platform for funktionel aflæsning af flere ionkanaler, der endogent udtrykkes i epitelcellelinjer.

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Denne måned i JoVE nummer 184
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