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Journal / Immunology and Infection / Anvendelse af Langsigtet kulturperler…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Anvendelse af Langsigtet kulturperler Interferon-γ Enzyme-linked immunospot Assay til Vurdering Effektorceller og Memory T celle responser i Cattle
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Anvendelse af Langsigtet kulturperler Interferon-γ Enzyme-linked immunospot Assay til Vurdering Effektorceller og Memory T celle responser i Cattle

Article DOI: 10.3791/52833-v • 15:58 min • July 11th, 2015
July 11th, 2015 •
1,2, 1, 3, 3, 4, 1, 1
1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, 3UK Veterinary Laboratories Agency, 4Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service, United States Department of Agriculture

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Langsigtet kulturperler interferon-γ enzymbundet immunospot assay anvendes som et mål for centrale hukommelse svarene og korrelerer med beskyttende anti-mycobakterielle vaccine reaktioner. Med dette assay er perifere mononukleære blodceller stimuleret med mycobakterielle antigener og interleukin-2 i 14 dage, muliggør differentiering og udvidelse af centrale hukommelse T-celler.

Tags

Immunologi , kvægtuberkulose CD4 T-celler vaccine.
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