JoVE Journal
Cancer Research
Cancer Research
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
The uneven loss of medium from microtiter plates affects the reproducibility of uniform multicellular tumor spheroid formation. Improving culture conditions to reduce significant medium loss will improve the reproducibility of spheroid formation and the results of spheroid-based assays using the liquid-overlay technique.