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Chapters
Summary
Please note that all translations are automatically generated.
该协议使用荧光记者和细胞分拣来简化巨噬细胞和T细胞系的敲击实验。两个质粒用于这些简化的敲击实验,即CRISPR/Cas9-和DsRed2表达质粒和同源重组供体质粒表达EBFP2,这是永久整合在 Rosa26 点在免疫细胞。