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Journal
/
Immunology and Infection
/
使用"桅杆细胞敲击
"小鼠分析体内
桅杆细胞的
功能
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Analyzing the Functions of Mast Cells
In Vivo
Using ‘
Mast Cell Knock-in
‘ Mice
Please note that all translations are automatically generated.
Click here for the English version.
使用"桅杆细胞敲击
"小鼠分析体内
桅杆细胞的
功能
DOI:
10.3791/52753-v
•
09:07 min
•
May 27, 2015
•
Nicolas Gaudenzio
,
Riccardo Sibilano
,
Philipp Starkl
,
Mindy Tsai
,
Stephen J. Galli
2
,
Laurent L. Reber
1
Department of Pathology
,
Stanford University School of Medicine
,
2
Department of Microbiology & Immunology
,
Stanford University School of Medicine
Chapters
00:05
Title
01:59
Generation and Characterization of Bone Marrow-derived Cultured Mast Cells (BMCMCs)
03:48
Engraftment of Mast Cell-deficient Mice with BMCMCs
05:30
Peritoneal Mast Cell Engraftment Analysis
06:50
Results: Representative BMCMC
In Vitro
Culture and
In Vivo
Engraftment
08:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了体
外
衍生桅杆细胞的生成方法,它们被移植到乳腺细胞缺乏的小鼠中,以及不同解剖部位对网状桅杆细胞的表型、数量和分布的分析。此协议可用于评估桅杆细胞
在体内的
功能。
Tags
Mast Cells
In Vivo
Knock-in Mice
Bone Marrow-derived Cultured Mast Cells
Adoptive Transfer
Anatomical Distribution
Host Defense
Allergic Reactions
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