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Journal / Immunology and Infection / Humaniseret Mediator Release Assay som en…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Humaniseret Mediator Release Assay som en udlæsning for Allergen Potency
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Humaniseret Mediator Release Assay som en udlæsning for Allergen Potency

Article DOI: 10.3791/62702-v • 10:22 min • June 29th, 2021
June 29th, 2021 •
1, 1, 1, 1
1Department of Biosciences, University of Salzburg

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Her præsenterer vi mægler frigivelse assay, ved hjælp af en rotte basophilic leukæmi celle linje transfected med den menneskelige IgE receptor, at simulere degranulation af effector celler typisk observeret i type 1 allergiske reaktioner. Denne metode undersøger den biologiske aktivitet af allergener på en meget følsom, reproducerbar og skræddersyet måde.

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Immunologi og infektion allergi RBL assay allergenicitet allergen mediator-release histamin IgE basophils AIT
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