JoVE Journal

Genetics

JoVE Genetics includes experimental approaches to study gene function, gene expression and regulation, epigenetics, genetic disorders, population genetics, and evolution.

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In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

J. Michael Harnish*¹, Samantha L. Deal*², Hsiao-Tuan Chao^1,3,4,5, Michael F. Wangler^1,2,4, Shinya Yamamoto^1,2,4,5

¹Department of Molecular and Human Genetics, Baylor College of Medicine, ²Program in Developmental Biology, Baylor College of Medicine, ³Department of Pediatrics, Section of Neurology and Developmental Neuroscience, Baylor College of Medicine, ⁴Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, ⁵Department of Neuroscience, Baylor College of Medicine

Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii

Titis A.K. Wardhani^1,2, Yuda Purwana Roswanjaya^1,2, Simon Dupin^1,3, Huchen Li^1,4, Sidney Linders¹, Marijke Hartog¹, Rene Geurts¹, Arjan van Zeijl¹

¹Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University & Research, ²Center of Technology for Agricultural Production, Agency for the Assessment and Application of Technology (BPPT), ³Department of Ecological Science, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, ⁴Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture

Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information

Julia Wang^1,2, Zhandong Liu^3,4, Hugo J. Bellen^1,4,5,6,7, Shinya Yamamoto^1,4,5,6

¹Program in Developmental Biology, Baylor College of Medicine, ²Medical Scientist Training Program, Baylor College of Medicine, ³Department of Pediatrics, Baylor College of Medicine, ⁴Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, ⁵Department of Molecular and Human Genetics, Baylor College of Medicine, ⁶Department of Neuroscience, Baylor College of Medicine, ⁷Howard Hughes Medical Institute, Baylor College of Medicine

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Carly V. Weiss^1,2, Julie N. Chuong³, Rachel B. Brem^1,3

¹Department of Plant and Microbial Biology, University of California Berkeley, ²Department of Biology, Stanford University, ³Buck Institute for Research on Aging

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Jory Lietard¹, Erika Schaudy¹, Kathrin Hölz¹, Dominik Ameur¹, Mark M. Somoza¹

¹Institute of Inorganic Chemistry, Faculty of Chemistry, University of Vienna

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Ravinder Singh¹

¹Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

Béatrice Colin¹, Nicolas Rocq², Benoit Deprez¹, Cyril Couturier¹

¹Institut Pasteur de Lille, Institut National de la Santé et de la Recherche Médicale (Inserm) U1177, Drugs and Molecules for Living Systems, Université de Lille, ²L'atelier du Développeur, Marquette lez Lille

Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System

Yukie Akutsu¹, Motomichi Doi¹, Koji Furukawa¹, Yuko Takagi¹

¹Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Yafeng Zhang^1,2, Xun Liao², Peihua Luo², Kui Peng², Wenyi Ye², Tengxiang Lian^1,2, Qibin Ma^1,2, Hai Nian^1,2

¹State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, ²Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Yurong Xin¹, Christina Adler¹, Jinrang Kim¹, Yueming Ding¹, Min Ni¹, Yi Wei¹, Lynn Macdonald¹, Haruka Okamoto¹

¹Regeneron Pharmaceuticals, Inc

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Lina Yu*¹, Wenxiu He*¹, Jie Xie*¹, Rui Guo*¹, Juan Ni², Xia Zhang³, Quishi Xu¹, Caifeng Wang¹, Qiuling Yue¹, Fangfang Li¹, Mengcheng Luo⁴, Bo Sun³, Lan Ye¹, Ke Zheng¹

¹State Key Laboratory of Reproductive Medicine, Nanjing Medical University, ²The Affiliated Hospital of Hangzhou Normal University, ³School of Life Science and Technology, ShanghaiTech University, ⁴Department of Tissue and Embryology, School of Basic Medical Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University

Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry

Johannes Korth^1,2, Helene Sertznig², Siegfried Moyrer², Adrian Atilla Nicolas Doevelaar^2,3, Timm Henning Westhoff³, Nina Babel³, Oliver Witzke⁴, Andreas Kribben¹, Ulf Dittmer², Marek Widera²

¹Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, ²Institute for Virology, University of Duisburg-Essen, University Hospital Essen, ³Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, ⁴Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr

Characterizing Mutational Load and Clonal Composition of Human Blood

Axel Rosendahl Huber¹, Freek Manders¹, Rurika Oka¹, Ruben van Boxtel¹

¹Princess Máxima Center for Pediatric Oncology

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

Olivia Gevedon¹, Harris Bolus¹, Shu Hui Lye¹, Keaton Schmitz¹, Jesualdo Fuentes-González¹, Kathryn Hatchell², Lyndsey Bley², Jason Pienaar¹, Carin Loewen², Stanislava Chtarbanova¹

¹Department of Biological Sciences, University of Alabama, ²Department of Genetics, University of Wisconsin-Madison

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Lauren Woodward¹, Pooja Gangras¹, Guramrit Singh¹

¹Department of Molecular Genetics, Center for RNA Biology, The Ohio State University

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Samantha B. Shelton¹, Blerta Xhemalce¹

¹Department of Molecular Biosciences, University of Texas at Austin

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Wilson Pak-Kin Lou*^1,2, Liqun Wang*³, Cheng Long¹, Lei Liu³, Ji-Feng Fei³

¹School of Life Sciences, South China Normal University, ²The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), ³Institute for Brain Research and Rehabilitation (IBRR), South China Normal University

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE

Nevena Cvetesic^1,2, Elena Pahita^1,2, Boris Lenhard^1,2,3

¹Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, ²MRC London Institute of Medical Sciences, ³Sars International Centre for Marine Molecular Biology, University of Bergen

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Linyuan Ma*¹, Lydia Jang*¹, Jian Chen², Jun Song¹, Dongshan Yang¹, Jifeng Zhang¹, Y. Eugene Chen¹, Jie Xu¹

¹Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, ²Celetrix LLC

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Snorre Gulla¹, Saima Nasrin Mohammad¹, Duncan John Colquhoun^1,2

¹Fish Health Research Group, Norwegian Veterinary Institute, ²University of Bergen

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Sophie Outh-Gauer¹, Jérémy Augustin¹, Marion Mandavit², Ophélie Grard², Thomas Denize¹, Marine Nervo¹, Charles Lépine¹, Marc Rassy², Eric Tartour^2,3, Cécile Badoual^1,2

¹Department of Pathology, Georges Pompidou European Hospital, Assistance publique - Hôpitaux de Paris (APHP), Paris Descartes University, ²Paris Cardiovascular Research Center (PARCC), Institut national de la santé et de la recherche médicale (Inserm) U970, ³Laboratory of Immunology, Georges Pompidou European Hospital, APHP, Paris Descartes University

Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer

Kristine M. Wadosky¹, Yanqing Wang¹, Xiaojing Zhang¹, David W. Goodrich¹

¹Department of Pharmacology & Therapeutics, Roswell Park Comprehensive Cancer Center

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

John A. Schmidt¹, Janice E. Knepper¹

¹Department of Biology, Villanova University

An Assay for Quantifying Protein-RNA Binding in Bacteria

Noa Katz*¹, Roni Cohen*¹, Orna Atar¹, Sarah Goldberg¹, Roee Amit^1,2

¹Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, ²Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology

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