JoVE Journal

Genetics

JoVE Genetics includes experimental approaches to study gene function, gene expression and regulation, epigenetics, genetic disorders, population genetics, and evolution.

1 2 3 4 5 6 7 8 9…14 › 321 VIDEO ARTICLES

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information

Adam Thrash¹, Marilyn L Warburton²

¹Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, ²USDA-ARS Corn Host Plant Resistance Research Unit, Mississippi State University

JoVE 61268

DNA Curtains Shed Light on Complex Molecular Systems During Homologous Recombination

Aviv Meir*¹, Muwen Kong*¹, Chaoyou Xue¹, Eric C. Greene¹

¹Department of Biochemistry & Molecular Biophysics, Columbia University

JoVE 61320

Next-generation Viral RNA/DNA in situ Hybridization Applications in Human Immunodeficiency Virus/Simian Immunodeficiency Virus Research

Catherine Brands¹, David Morcock¹, Jacob Estes², Claire Deleage¹

¹AIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., ²Vaccine and Gene Therapy Institute and Oregon National Primate Research Center (ONPRC), Oregon Health and Science University (OHSU)

JoVE 60318

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Gabrielle J. Benitez¹, Kosaku Shinoda^1,2,3

¹Departments of Medicine, Albert Einstein College of Medicine, ²Departments of Molecular Pharmacology, Albert Einstein College of Medicine, ³Fleischer Institute of Diabetes and Metabolism

JoVE 61230

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

Keerat Kaur^1,2,3, Nishat Sultana^1,2,3, Yoav Hadas^1,2,3, Ajit Magadum^1,2,3, Mohammad Tofael Kabir Sharkar^1,2,3, Elena Chepurko^1,2,3, Lior Zangi^1,2,3

¹Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, ²Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai

JoVE 60832

Methods Collection

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Andi M. Wilson^1,2, Brenda D. Wingfield^1,2

¹Department of Biochemistry, Genetics & Microbiology, University of Pretoria, ²Forestry & Agricultural Biotechnology Institute (FABI), University of Pretoria

JoVE 61367

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Yelena Levin*¹, Keyur Talsania*^1,2, Bao Tran¹, Jyoti Shetty¹, Yongmei Zhao^1,2, Monika Mehta¹

¹NCI CCR Sequencing Facility, Frederick National Laboratory for Cancer Research, ²Advanced Biomedical and Computational Sciences, Frederick National Laboratory for Cancer Research

JoVE 61060

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Sumana Sharma^1,2, Gavin J. Wright¹

¹Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, ²EMBL-EBI, Wellcome Genome Campus

JoVE 60803

Enforced Activation of Enhancer RNAs In Situ through the dCas9 Synergistic Activation Mediator System

Zian Liao*^1,2, Joo-Hyung Lee*¹, Joanna Krakowiak¹, Ruoyu Wang^1,2, Wenbo Li^1,2

¹Department of Biochemistry and Molecular Biology, McGovern Medical School, University of Texas Health Science Center, ²Graduate School of Biomedical Sciences, University of Texas MD Anderson Cancer Center and UTHealth

JoVE 61460

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

Laetitia Chauve*¹, Jérémie Le Pen*^2,3,5, Francesca Hodge¹, Pia Todtenhaupt¹, Laura Biggins¹, Eric A. Miska^2,3,4, Simon Andrews¹, Olivia Casanueva¹

¹Babraham Institute, ²Gurdon Institute, University of Cambridge, ³Department of Genetics, University of Cambridge, ⁴Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute, ⁵Laboratory of Virology and Infectious Disease, The Rockefeller University

JoVE 61132

Methods Collection

Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus

Michelle Bui¹, Ming Li¹, Robyn R Raban¹, Nannan Liu², Omar S Akbari^1,3

¹Section of Cell and Developmental Biology, University of California, San Diego, ²Department of Entomology and Plant Patholog, Auburn University, ³Tata Institute for Genetics and Society, University of California, San Diego

JoVE 61375

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification

Xianju Bi^1,2, Xuechun Zhang¹, Xiaohua Shen¹

¹Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences, Tsinghua University, ²Department of Molecular Biology, University of Texas Southwestern Medical Center

JoVE 60730

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Jos G.A. Smits*¹, Jieqiong Qu*¹, Hanna Niehues², Huiqing Zhou^1,3

¹Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands, ²Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands, ³Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands

JoVE 60905

Methylated RNA Immunoprecipitation Assay to Study m⁵C Modification in Arabidopsis

Eleftheria Saplaoura*¹, Valentina Perrera*², Friedrich Kragler¹

¹Max Planck Institute of Molecular Plant Physiology, ²Department of Molecular Medicine, Medical School, University of Padua

JoVE 61231

Direct Liquid-Culture Screening for Evaluating the Production of Heterologous Proteins Using an Auxotrophic Mutant of Aspergillus Oryzae

Satoru Shinkawa¹, Shigenobu Mitsuzawa¹

¹Honda Research Institute Japan Co., Ltd.

JoVE 61219

Introducing Point Mutations into Human Pluripotent Stem Cells using Seamless Genome Editing

Yu Wang¹, Andrew J. H. Smith¹, David C. Hay¹

¹MRC Centre for Regenerative Medicine, University of Edinburgh

JoVE 61152

Methods Collection

Exon Skipping in Directly Reprogrammed Myotubes Obtained from Human Urine-Derived Cells

Hotake Takizawa¹, Mitsuto Sato¹, Yoshitsugu Aoki¹

¹Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry

JoVE 60840

Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)

Tian V. Tian^1,2,3, Enrique Vidal^1,2, Thomas Graf^1,2, Grégoire Stik^1,2

¹Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, ²Universitat Pompeu Fabra, ³Vall d'Hebron Institute of Oncology

JoVE 60960

Engineering Intracellular Protein Sensors in Mammalian Cells

Marina Duhkinova¹, Claudia Crina¹, Ron Weiss², Velia Siciliano¹

¹Istituto Italiano di Tecnologia, ²Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology

JoVE 60878

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Hirofumi Nishizono*^1,2,3, Mohamed Darwish*^4,5, Hideki Uosaki^6,7, Nanami Masuyama^8,9,10, Motoaki Seki^8,11, Hiroyuki Abe³, Nozomu Yachie^8,9,10,12,13, Ryohei Yasuda¹

¹Max Planck Florida Institute for Neuroscience, ²Life Science Research Center, University of Toyama, ³Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, ⁴Graduate School of Innovative Life Science, University of Toyama, ⁵Department of Biochemistry, Faculty of Pharmacy, Cairo University, ⁶Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, ⁷Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, ⁸Synthetic Biology Division, Research Center for Advanced Science and Technology, University of Tokyo, ⁹Institute for Advanced Biosciences, Keio University, ¹⁰Graduate School of Media and Governance, Keio University, ¹¹Department of Molecular Oncology, Graduate School of Medicine, Chiba University, ¹²Department of Biological Sciences, School of Science, University of Tokyo, ¹³College of Arts and Sciences, University of Tokyo

JoVE 60808

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Krzysztof J. Skowronek¹, Matthias Bochtler¹

¹International Institute of Molecular and Cell Biology, Warsaw (IIMCB)

JoVE 60807

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Cheryl A. Hobbs*¹, Leslie Recio¹, John Winters¹, Kristine L. Witt²

¹Toxicology Program, ILS, Inc., ²Division of the National Toxicology Program, National Institute of Environmental Health Sciences

JoVE 59955

A Screening Method for Identification of Heterochromatin-Promoting Drugs Using Drosophila

Lin Zhang¹, Kenny Dao¹, Angela Kang¹, Andre C. Loyola¹, Robin Shang¹, Jinghong Li¹, Willis X. Li¹

¹Department of Medicine, University of California San Diego

JoVE 60917

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Bas B. Oude Munnink¹, David F. Nieuwenhuijse¹, Reina S. Sikkema¹, Marion Koopmans¹

¹ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research, Erasmus University Medical Center

JoVE 60906

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Biochemistry

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