JoVE Journal

Genetics

JoVE Genetics includes experimental approaches to study gene function, gene expression and regulation, epigenetics, genetic disorders, population genetics, and evolution.

1 2 3 4 5 6 7 8 9 10 › 231 VIDEO ARTICLES

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Tiffany Kaul¹, Maria E. Morales¹, Emily Smither¹, Melody Baddoo^1,2, Victoria P. Belancio^1,3, Prescott Deininger^1,4

¹Tulane Cancer Center, Tulane University, ²Department of Pathology, Tulane University, ³Department of Structural and Cellular Biology, Tulane University, ⁴Department of Epidemiology, Tulane University

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Zachary J. Heins^1,2, Christopher P. Mancuso^1,2, Szilvia Kiriakov^1,3, Brandon G. Wong^1,2, Caleb J. Bashor⁴, Ahmad S. Khalil^1,2,5

¹Biological Design Center, Boston University, ²Department of Biomedical Engineering, Boston University, ³Program in Molecular Biology, Cell Biology and Biochemistry, Boston University, ⁴Department of Bioengineering, Rice University, ⁵Wyss Institute for Biologically Inspired Engineering, Harvard University

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

Siming Liu^1,2, Mikako Harata^1,2, Joseph A. Promes^1,2, Anthony J. Burand^2,3, James A. Ankrum^2,3, Yumi Imai^1,2

¹Department of Internal Medicine, Carver College of Medicine, University of Iowa, ²Fraternal Order of Eagles Diabetes Research Center, University of Iowa, ³Roy J. Carver Department of Biomedical Engineering, University of Iowa

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

Jeff A. Shaw¹, Travis J. Bourret¹

¹Department of Medical Microbiology and Immunology, Creighton University School of Medicine

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Melvin Noe Gonzalez¹, Joan W. Conaway^1,2, Ronald C. Conaway^1,2

¹Stowers Institute for Medical Research, ²Department of Biochemistry and Molecular Biology, Kansas University Medical Center

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies

Simone Chiola¹, Manuela Santo¹, Antonello Mallamaci¹

¹Laboratory of Cerebral Cortex Development, SISSA

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Takahiro Yamakawa¹, Keiichi Itakura¹

¹Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Ana Korosec*¹, Sophie Frech*¹, Beate M. Lichtenberger¹

¹Skin, Endothelium Research Division, Department of Dermatology, Medical University of Vienna

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

Yuyang Wang*¹, Weidi Xu*^1,2, Huancheng Guo¹, Wenjie Gong¹, Biao He¹, Zhongzhong Tu¹, Changchun Tu^1,3, Ye Feng¹

¹Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, ²Jilin Agricultural University, ³Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Screening Cotton Genotypes for Reniform Nematode Resistance

John E. Erpelding¹, Salliana R. Stetina¹

¹Crop Genetics Research Unit, Agricultural Research Service, United States Department of Agriculture

Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes

Kelsey R. Timme¹, Jennifer R. Wood¹

¹Department of Animal Science, University of Nebraska-Lincoln

Manipulation of Gene Function in Mexican Cavefish

Bethany A. Stahl^1,2, James B. Jaggard^1,2, Jacqueline S.R. Chin^1,2, Johanna E. Kowalko^2,3, Alex C. Keene^1,2, Erik R. Duboué^2,3

¹Department of Biological Sciences, Florida Atlantic University, ²Jupiter Life Science Initiative, Florida Atlantic University, ³Harriet L. Wilkes Honors College, Florida Atlantic University

Methods Collection

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Vadir López-Salmerón*¹, Ann-Kathrin Schürholz*², Zhenni Li², Theresa Schlamp¹, Christian Wenzl³, Jan U. Lohmann³, Thomas Greb¹, Sebastian Wolf²

¹Department of Developmental Physiology, Centre for Organismal Studies (COS) Heidelberg, ²Department of Cell Biology, Centre for Organismal Studies (COS) Heidelberg, ³Department of Stem Cell Biology, Centre for Organismal Studies (COS) Heidelberg

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Marie A. Brunet^1,2, Xavier Roucou^1,2

¹Department of Biochemistry, Université de Sherbrooke, ²PROTEO, Quebec Network for Research on Protein Function, Structure, and Engineering

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Kaiwen Ivy Liu¹, Norfala-Aliah Binte Sutrisnoh¹, Yuanming Wang^1,2, Meng How Tan^1,2

¹Genome Institute of Singapore, Agency for Science Technology and Research, ²School of Chemical and Biomedical Engineering, Nanyang Technological University

Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice

Xuan Su*^1,2, Yan Shen*¹, Yue Jin*^1,2, Meng Jiang², Neal Weintraub¹, Yaoliang Tang¹

¹Vascular Biology Center, Medical College of Georgia, Augusta University, ²Renji Hospital, School of Medicine, Shanghai Jiaotong University

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

Elizabeth C. Bondy¹, Martha S. Hunter²

¹Graduate Interdisciplinary Program in Entomology and Insect Science, University of Arizona, ²Department of Entomology, University of Arizona

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Vincenzo De Iuliis¹, Sebastiano Ursi¹, Alfonso Pennelli², Marika Caruso², Sabrina Capodifoglio², Antonio Marino¹, Vincenzo Flati³, Gianfranco Vitullo¹, Elena Toniato², Iole Robuffo⁴, Stefano Martinotti^1,2

¹SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, ²Department of Medical, Oral and Biotechnological Sciences, University of Chieti, ³Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, ⁴CNR - Institute of Molecular Genetics, Section of Chieti

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Huacheng Luo¹, Amin Sobh², Christopher D. Vulpe², Edmond Brewer¹, Sinisa Dovat¹, Yi Qiu³, Suming Huang¹

¹Department of Pediatrics, Pennsylvania State University College of Medicine, ²Department of Physiological Sciences, University of Florida, ³Department of Anatomy and Cell Biology, University of Florida

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Lidia Tagliafierro^1,2, Ekaterina Ilich³, Malik Moncalvo³, Jeffrey Gu¹, Ahila Sriskanda^1,2, Carole Grenier⁴, Susan K. Murphy⁴, Ornit Chiba-Falek^1,2, Boris Kantor³

¹Department of Neurology, Duke University Medical Center, ²Center for Genomic and Computational Biology, Duke University Medical Center, ³Viral Vector Core, Duke University Medical Center, ⁴Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Duke University Medical Center

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Nicole A. Vander Schaaf¹, Shirley Oghamian², Jin-A Park¹, Liang Kang¹, Peter W. Laird¹, Kwang-Ho Lee¹

¹Center for Epigenetics, Van Andel Research Institute, ²Amgen

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

Seth A. Bennett^1,2, Samantha N. Cobos^1,3, Marcella Meykler¹, Michel Fallah¹, Navin Rana¹, Karen Chen¹, Mariana P. Torrente^1,4

¹Chemistry Department, Brooklyn College, ²Ph.D. Program in Biochemistry, Graduate Center of the City University of New York, ³Ph.D. Program in Chemistry, Graduate Center of the City University of New York, ⁴Ph.D. Programs in Chemistry, Biochemistry, and Biology, Graduate Center of the City University of New York

ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes

Hannah D. Rickner¹, Sheng-Yong Niu^1,2, Christine S. Cheng^1,3

¹Department of Biology, Boston University, ²Department of Computer Science and Engineering, University of California San Diego, ³Bioinformatics Program, Boston University

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Yan Yan*¹, Chi-Chih Chang*¹, Morten T. Venø^1,2, Colin R. Mothershead¹, Junyi Su¹, Jørgen Kjems^1,2

¹The Interdisciplinary Nanoscience Centre, Aarhus University, ²Department of Molecular Biology and Genetics, Aarhus University

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