JoVE Journal

Genetics

JoVE Genetics covers a wide array of experimental approaches used to investigate gene function, gene regulation, epigenetics, genetic disorders, population genetics and evolution.

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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Desiree-Faye Kaixin Toh*¹, Kiran M. Patil*¹, Gang Chen¹

¹Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University

JoVE 56221

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster

Soda Balla Diop¹, Ryan T. Birse¹, Rolf Bodmer¹

¹Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute

JoVE 56029

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Jakob Frimodt-Møller¹, Godefroid Charbon¹, Karen A. Krogfelt², Anders Løbner-Olesen¹

¹Department of Biology, Section for Functional Genomics and Center for Bacterial Stress Response and Persistence (BASP), University of Copenhagen, ²Department of Microbiology and Infection Control, Statens Serum Institut

JoVE 55946

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Ashley H. Ramsawhook*¹, Lara C. Lewis*¹, Maria Eleftheriou¹, Abdulkadir Abakir¹, Paulina Durczak¹, Robert Markus², Seema Rajani², Nicholas R.F. Hannan¹, Beth Coyle³, Alexey Ruzov¹

¹Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, ²School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, ³Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham

JoVE 56318

Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification (ADCI) and Dose Estimation

Ben Shirley*¹, Yanxin Li*¹, Joan H.M. Knoll^1,2, Peter K. Rogan^1,3

¹CytoGnomix Inc., ²Department of Pathology and Laboratory Medicine, Western University, ³Department of Biochemistry, Western University

JoVE 56245

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

Rizwan Rehimi¹, Michaela Bartusel¹, Francesca Solinas², Janine Altmüller², Alvaro Rada-Iglesias^1,3

¹Center for Molecular Medicine Cologne (CMMC), University of Cologne, ²Cologne Center for Genomics (CCG), University of Cologne, ³Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), Research Center and Systems Biology of Ageing Cologne, University of Cologne

JoVE 56186

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Natalia Rivera-Torres^1,2, Eric B. Kmiec^1,2

¹Gene Editing Institute, Helen F. Graham Cancer Center and Research Institute, Christiana Care Health Services, ²Department of Medical Sciences, University of Delaware

JoVE 56195

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

Dhaval S Patel*¹, Giovanni Diana*¹, Eugeni V. Entchev¹, Mei Zhan^2,3,4, Hang Lu^2,3,4, QueeLim Ch'ng¹

¹Centre for Developmental Neurobiology, King's College London, ²Interdisciplinary Bioengineering Graduate Program, Georgia Institute of Technology, ³Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, ⁴School of Chemical & Biomolecular Engineering, Georgia Institute of Technology

JoVE 56292

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Stephen D. Willis¹, A. K. M. Nawshad Hossian², Nathan Evans², Mark J. Hickman²

¹Department of Molecular Biology, Rowan School of Osteopathic Medicine, ²Department of Biological Sciences, Rowan University

JoVE 56226

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Marisa E. Miller*^1,2, Katie L. Liberatore*^1,3, Shahryar F. Kianian^1,3

¹Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service, ²Department of Horticultural Science, University of Minnesota, ³Department of Plant Pathology, University of Minnesota

JoVE 55528

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Aymric Kisserli^1,2, Thierry Tabary^1,2, Jacques Henri Max Cohen^1,2, Valérie Duret^1,2, Rachid Mahmoudi^3,4

¹Department of Immunology, Reims University Hospitals, Robert Debré Hospital, ²Faculty of Medicine, LRN EA 4682, University of Reims Champagne-Ardenne, ³Department of Internal Medicine and Geriatrics, Reims University Hospitals, Maison Blanche Hospital, ⁴Faculty of Medicine, EA 3797, University of Reims Champagne-Ardenne

JoVE 56012

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation

Pingyu Zhang*¹, Andres Rojas*¹, Boris Blechacz¹

¹Department of Gastroenterology, Hepatology and Nutrition, The University of Texas MD Anderson Cancer Center

JoVE 55689

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Keiji Hirai¹, Hiromichi Yoshizawa², Toshimi Imai², Yusuke Igarashi², Ichiro Hirahara², Susumu Ookawara², Kenichi Ishibashi³, Yoshiyuki Morishita¹

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ²Division of Nephrology, Department of Internal Medicine, Jichi Medical University, ³Department of Medical Physiology, Meiji Pharmaceutical University

JoVE 55505

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Mohamed I. Hassan*¹, Fern R. McSorley*¹, Kinya Hotta², Christopher N. Boddy¹

¹Department of Chemistry and Biomolecular Sciences, University of Ottawa, ²School of Biosciences, The University of Nottingham Malaysia Campus

JoVE 55187

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

Bang Shen¹, Robin H. Powell², Michael S. Behnke²

¹State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, ²Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University

JoVE 55185

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

Benedetto Daniele Giaimo¹, Francesca Ferrante¹, Tilman Borggrefe¹

¹Institute of Biochemistry, University of Giessen

JoVE 55907

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Erin L. Sternburg¹, Kristen C. Dias¹, Fedor V. Karginov¹

¹Cell Biology and Neuroscience, University of California, Riverside

JoVE 55903

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Santhilal Subhash¹, Meena Kanduri²

¹Department of Medical Genetics, Institute of Biomedicine, Gothenburg University, ²Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Gothenburg University

JoVE 55773

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Shi-Yuan Li¹, Guo-Ping Zhao¹, Jin Wang¹

¹Institute of Plant Physiology and Ecology, Shanghai Insititutes for Biological Sciences, Chinese Academy of Sciences

JoVE 55775

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Gajendra W. Suryawanshi*^1,2, Song Xu*³, Yiming Xie¹, Tom Chou³, Namshin Kim⁴, Irvin S. Y. Chen^1,5, Sanggu Kim⁶

¹UCLA AIDS Institute, University of California at Los Angeles (UCLA), ²Department of Microbiology, Immunology, & Molecular Genetics, University of California at Los Angeles (UCLA), ³Departments of Biomathematics and Mathematics, University of California at Los Angeles (UCLA), ⁴Personalized Genomic Medicine Research Center, Division of Strategic Research Groups, Korea Research Institute of Bioscience and Biotechnology, ⁵Department of Medicine, University of California at Los Angeles (UCLA), ⁶Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University (OSU)

JoVE 55812

Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs

Scott E. Gold¹, Zahi Paz², María D. García-Pedrajas³, Anthony E. Glenn¹

¹Toxicology and Mycotoxin Research Unit, NPRC, USDA-ARS, ²Hazera Seeds LTD, Brurim, ³Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” - Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”