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JoVE Journal

Genetics

JoVE Genetics includes experimental approaches to study gene function, gene expression and regulation, epigenetics, genetic disorders, population genetics, and evolution.

1 2 3 4 5 6 7 8 9…14 › 336 VIDEO ARTICLES

Optimized High Quality DNA Extraction from Formalin-Fixed Paraffin-Embedded Human Atherosclerotic Lesions

Sabine Bauer^1,2,4, Jessica Pauli^1,2, Jaroslav Pelisek^1,3

¹Department for Vascular and Endovascular Surgery, Klinikum rechts der Isar, Technical University Munich, ²partner site Munich Heart Alliance, Deutsches Zentrum für Herz-Kreislaufforschung (DZHK), ³Department of Vascular Surgery, University Hospital Zurich, ⁴Department of Experimental Cardiology, German Heart Centre, Technical University Munich

Methods Collection

Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

Kayli Sieber¹, Maya Saar¹, Comzit Opachaloemphan², Matthew Gallitto³, Huan Yang², Hua Yan^1,4

¹Department of Biology, University of Florida, ²Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, ³Department of Radiation Oncology, Columbia University Medical Center, ⁴Center for Smell and Taste, University of Florida

Methods Collection

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Adriana Adolfi^1,2, Amy Lynd¹, Gareth J. Lycett¹, Anthony A. James^2,3

¹Vector Biology Department, Liverpool School of Tropical Medicine, ²Department of Microbiology & Molecular Genetics, University of California, ³Department of Molecular Biology & Biochemistry, University of California

Methods Collection

R-Loop Analysis by Dot-Blot

Prisila Ramirez¹, Robert J. Crouch², Vivian G. Cheung^1,3,4, Christopher Grunseich⁵

¹Life Sciences Institute, University of Michigan, Ann Arbor, ²Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, ³Department of Pediatrics, University of Michigan, Ann Arbor, ⁴Howard Hughes Medical Institute, ⁵Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health

Methods Collection

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Amy Smith¹, Mary Bergwell*¹, Ellie Smith*¹, Danita Mathew*¹, Jyoti Iyer¹

¹Department of Chemistry and Biochemistry, The University of Tulsa

Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Isabelle Louradour¹, Kashinath Ghosh¹, Ehud Inbar¹, David L. Sacks¹, Channa Aluvihare², Robert A. Harrell II²

¹Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, ²University of Maryland Insect Transformation Facility, The Institute for Bioscience and Biotechnology Research

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Sampath Kumar Loganathan¹, Ahmad Malik^1,2, Ellen Langille^1,2, Chen Luxenburg³, Daniel Schramek^1,2

¹Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, ²Department of Molecular Genetics, University of Toronto, ³Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

Methods Collection

An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level

Payam Haftbaradaran Esfahani¹, Ralph Knöll^1,2

¹Department of Medicine, Integrated Cardio Metabolic Centre (ICMC), Heart and Vascular Theme, Karolinska Institutet, ²Bioscience Cardiovascular, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca

Isolation of High Purity Tissues from Developing Barley Seeds

Martin Kovacik¹, Anna Nowicka^1,2, Ales Pecinka¹

¹Czech Acad Sci, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, ²The Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology

Gene Mining and Sequence Analysis of Purine Nucleosidase Based on RNA-Seq

Fenfang Wu¹, Shiping Hu², Yun Ran², Xiaoni Chen¹, Shan Lin¹

¹Department of Central Laboratory, Shenzhen Hospital, Beijing University of Chinese Medicine, ²Department of Hepatopathy, Shenzhen Hospital, Beijing University of Chinese Medicine

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Christie Dix¹, Susan Sgro¹, Anup Patel¹, Carly Perrotta¹, Noor Eldabagh¹, Katherine L. Lomauro¹, Fredy W. Miguez¹, Prianka Chohan¹, Charmi Jariwala¹, James T. Arnone¹

¹Department of Biology, William Paterson University

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

Katsunori Yanai¹, Shohei Kaneko¹, Hiroki Ishii¹, Akinori Aomatsu^1,2, Kiyonori Ito¹, Keiji Hirai¹, Susumu Ookawara¹, Kenichi Ishibashi³, Yoshiyuki Morishita¹

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ²Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ³Department of Medical Physiology, Meiji Pharmaceutical University

Methods Collection

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

Tadej Pajič^1,2, Tanja Belčič Mikič¹, Helena Podgornik^1,3, Jurka Klun¹, Sandra Šućurović¹, Samo Zver¹, Matjaz Sever¹

¹Department of Hematology, University Medical Centre Ljubljana, ²Clinical Institute for Genomic Medicine, University Medical Centre Ljubljana, ³Faculty of Pharmacy, University of Ljubljana

High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)

Kaitlin N. Montanera^1,2, Ho Sung Rhee^1,2

¹Department of Cell and Systems Biology, University of Toronto, ²Department of Biology, University of Toronto

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Ning Zhang^1,2, Shundi Shi², Barney Yoo³, Xiaohong Yuan¹, Wenjia Li⁴, Shenglong Zhang¹

¹Department of Biological and Chemical Sciences, New York Institute of Technology, ²Department of Chemical Engineering, Columbia University, ³Department of Chemistry, Hunter College, City University of New York, ⁴Department of Computer Science, New York Institute of Technology

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information

Adam Thrash¹, Marilyn L Warburton²

¹Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, ²USDA-ARS Corn Host Plant Resistance Research Unit, Mississippi State University

DNA Curtains Shed Light on Complex Molecular Systems During Homologous Recombination

Aviv Meir*¹, Muwen Kong*¹, Chaoyou Xue¹, Eric C. Greene¹

¹Department of Biochemistry & Molecular Biophysics, Columbia University

Methods Collection

Next-generation Viral RNA/DNA in situ Hybridization Applications in Human Immunodeficiency Virus/Simian Immunodeficiency Virus Research

Catherine Brands¹, David Morcock¹, Jacob Estes², Claire Deleage¹

¹AIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., ²Vaccine and Gene Therapy Institute and Oregon National Primate Research Center (ONPRC), Oregon Health and Science University (OHSU)

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Gabrielle J. Benitez¹, Kosaku Shinoda^1,2,3

¹Departments of Medicine, Albert Einstein College of Medicine, ²Departments of Molecular Pharmacology, Albert Einstein College of Medicine, ³Fleischer Institute of Diabetes and Metabolism

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

Keerat Kaur^1,2,3, Nishat Sultana^1,2,3, Yoav Hadas^1,2,3, Ajit Magadum^1,2,3, Mohammad Tofael Kabir Sharkar^1,2,3, Elena Chepurko^1,2,3, Lior Zangi^1,2,3

¹Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, ²Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai

Methods Collection

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Andi M. Wilson^1,2, Brenda D. Wingfield^1,2

¹Department of Biochemistry, Genetics & Microbiology, University of Pretoria, ²Forestry & Agricultural Biotechnology Institute (FABI), University of Pretoria

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Yelena Levin*¹, Keyur Talsania*^1,2, Bao Tran¹, Jyoti Shetty¹, Yongmei Zhao^1,2, Monika Mehta¹

¹NCI CCR Sequencing Facility, Frederick National Laboratory for Cancer Research, ²Advanced Biomedical and Computational Sciences, Frederick National Laboratory for Cancer Research

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Sumana Sharma^1,2, Gavin J. Wright¹

¹Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, ²EMBL-EBI, Wellcome Genome Campus

Enforced Activation of Enhancer RNAs In Situ through the dCas9 Synergistic Activation Mediator System

Zian Liao*^1,2, Joo-Hyung Lee*¹, Joanna Krakowiak¹, Ruoyu Wang^1,2, Wenbo Li^1,2

¹Department of Biochemistry and Molecular Biology, McGovern Medical School, University of Texas Health Science Center, ²Graduate School of Biomedical Sciences, University of Texas MD Anderson Cancer Center and UTHealth

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