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Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes

Article DOI: 10.3791/1790-v • 08:46 min • February 15th, 2010
February 15th, 2010 •
1, 1
1STED Microscopy of Synaptic Function, European Neuroscience Institute Göttingen

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FM dyes have been of invaluable help in the understanding of synaptic dynamics. FMs are normally followed under the fluorescent microscope during different stimulation conditions. However, photoconversion of FM dyes combined with electron microscopy allows the visualization of distinct synaptic vesicle pools, among other ultrastructure components, in synaptic boutons.

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Synaptic Vesicle Pools Photoconversion Styryl Dyes Exocytosis Endocytosis Vesicle Recycling Fluorescently Labeled Markers Optical Techniques FM Dye Vesicle Labeling Nerve Stimulation Dye Loading Exocytosis Process Fluorescence Monitoring
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