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Modifizierten Hefe-Zwei-Hybrid-System, um Proteine ​​interagieren mit dem Growth Factor Progranulin Identifizieren

Article DOI: 10.3791/3562-v • 07:57 min • January 17th, 2012
January 17th, 2012 •
1, 1, 1,2
1Department of Orthopaedic Surgery, NYU Hospital for Joint Diseases, 2Department of Cell Biology, New York University School of Medicine

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Wir haben die konventionelle Hefe-Zwei-Hybrid-Screening, ein wirksames Instrument bei der Identifizierung genetischer Protein-Interaktion verändert. Diese Modifikation deutlich verkürzt den Prozess, reduziert den Arbeitsaufwand und vor allem, reduziert die Anzahl von Fehlalarmen. Darüber hinaus ist dieser Ansatz reproduzierbar und zuverlässig.

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Molecular Biology Ausgabe 59 Modified Hefe-Zwei-Hybrid-Screen PGRN TNFR2 Entzündungen Autoimmunkrankheiten
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