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Analyse der Translation Initiation Während Stress-Bedingungen durch Polysomprofile Profiling
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Analyse der Translation Initiation Während Stress-Bedingungen durch Polysomprofile Profiling

Article DOI: 10.3791/51164-v • 10:59 min • May 19th, 2014
May 19th, 2014 •
*1,2, *1,2, 1,2
1Department of Molecular Biology, Medical Biochemistry, and Pathology, Faculty of Medicine, Laval University, 2CHU de Quebec Research Center
* These authors contributed equally

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Hier beschreiben wir ein Verfahren, um Veränderungen in der mRNA-Translationsinitiation eukaryontischer Zellen in Reaktion auf Stress-Bedingungen zu untersuchen. Dieses Verfahren basiert auf der Geschwindigkeit Trennung auf Saccharosegradienten übersetzen Ribosomen nicht übersetzen Ribosomen basiert.

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Cellular Biology Ausgabe 87 Übersetzung Initiation Polysom ​​Profil Saccharose-Gradienten Protein-und RNA-Isolierung Stress-Bedingungen
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