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Bioengineering

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Synthèse des bactériophages infectieuses dans un e. coli -basé système d’Expression acellulaire

Article DOI: 10.3791/56144-v • 11:33 min • August 17th, 2017
August 17th, 2017 •
1, 2, 1, 2, 1
1School of Physics and Astronomy, University of Minnesota, 2Department of Diagnostic and Biological Sciences and Institute for Molecular Virology, University of Minnesota

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Une nouvelle génération de plates-formes de transcription-traduction acellulaire a été conçue pour construire des systèmes biochimiques in vitro par le biais de l’exécution des circuits de gène. Dans cet article, nous décrivons comment bactériophages MS2, ΦΧ174 et T7, sont synthétisés à partir de leur génome en utilisant un système tous les e. coli acellulaire TXTL.

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Numéro 126 traduction transcription acellulaire (TXTL) bio-ingénierie bactériophage e. coli essai de plaque amplification phage purification du génome phagique biologie synthétique
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