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Journal / Bioengineering / Synthese von infektiösen Bakteriophagen in einem…
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Bioengineering

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JoVE Journal Bioengineering
Synthese von infektiösen Bakteriophagen in einem E. Coli -basierte zellfreie Expressionssystems
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Synthese von infektiösen Bakteriophagen in einem E. Coli -basierte zellfreie Expressionssystems

Article DOI: 10.3791/56144-v • 11:33 min • August 17th, 2017
August 17th, 2017 •
1, 2, 1, 2, 1
1School of Physics and Astronomy, University of Minnesota, 2Department of Diagnostic and Biological Sciences and Institute for Molecular Virology, University of Minnesota

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Eine neue Generation von zellfreien Transkription-Übersetzung Plattformen wurde entwickelt, um die biochemischen Systemen in Vitro durch die Ausführung des Gens Schaltungen aufbauen. In diesem Artikel beschreiben wir, wie aus ihrem Genom mit einem alle E. Coli zellfreie TXTL System Bakteriophagen MS2, ΦΧ174 und T7, synthetisiert werden.

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Biotechnik Ausgabe 126 zellfreie Transkription-Übersetzung (TXTL) Bakteriophagen E. Coli Plaque-Assay Phagen-Verstärkung Phagen Genom Reinigung synthetische Biologie
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