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Journal / Biology / Isolierung von hochwertigen atrialen und…
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Biology

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Isolierung von hochwertigen atrialen und ventrikulären Myozyten der Maus für die simultane Messung von Ca2+ Transienten und L-Typ Calciumstrom
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Isolierung von hochwertigen atrialen und ventrikulären Myozyten der Maus für die simultane Messung von Ca2+ Transienten und L-Typ Calciumstrom

Article DOI: 10.3791/61964-v • 06:22 min • November 3rd, 2020
November 3rd, 2020 •
*1,2,3, *1,2,3, 1,2, *1,2,3, *4,5,6
1Department of Medicine I, University Hospital Munich, Campus Großhadern, Ludwig-Maximilians University Munich (LMU), 2Partner Site Munich, Munich Heart Alliance (MHA), DZHK (German Centre for Cardiovascular Research), 3Walter Brendel Center of Experimental Medicine, Ludwig-Maximilians University Munich (LMU), 4Institute of Pharmacology and Toxicology, University Medical Center Göttingen, 5Partner Site Göttingen, DZHK (German Centre for Cardiovascular Research), 6Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen
* These authors contributed equally

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Mausmodelle ermöglichen es, Schlüsselmechanismen der Arrhythmogenese zu untersuchen. Zu diesem Zweck sind qualitativ hochwertige Kardiomyozyten notwendig, um Patch-Clamp-Messungen durchzuführen. In dieser Arbeit wird eine Methode zur Isolierung muriner atrialer und ventrikulärer Myozyten mittels retrograder enzymbasierter Langendorff-Perfusion beschrieben, die simultane Messungen von Calcium-Transienten und L-Typ-Calciumstrom ermöglicht.

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Diesen Monat in JoVE Ausgabe 165 Arrhythmie murine Myozytenisolierung Langendorff-Perfusion L-Typ Calciumstrom Calcium Transient Patch Clamp
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