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Phospholipid-Mediator-induzierte Transformation in dreidimensionalen Kulturen

Article DOI: 10.3791/64146-v • 08:02 min • July 27th, 2022
July 27th, 2022 •
1, 1,2, 1,3, 1
1Biology Department, Indian Institute of Science Education and Research, 2Department of Biology, Center for Genomics and Systems Biology, New York University, 3Enveda Therapeutics India Pvt Ltd.

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Das vorliegende Protokoll beschreibt den Aufbau von 3D-On-Top-Kulturen einer nicht transformierten Brustepithelzelllinie, MCF10A, die modifiziert wurde, um die durch den Thrombozytenaktivierungsfaktor (PAF) induzierte Transformation zu untersuchen. Immunfluoreszenz wurde verwendet, um die Transformation zu bewerten und wird ausführlich diskutiert.

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Krebsforschung Ausgabe 185 Phospholipid-Mediator MCF10A-Zellen dreidimensionale Kulturen Transformation PAF
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