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Transformación inducida por mediadores fosfolípidos en cultivos tridimensionales
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Transformación inducida por mediadores fosfolípidos en cultivos tridimensionales

Article DOI: 10.3791/64146-v • 08:02 min • July 27th, 2022
July 27th, 2022 •
1, 1,2, 1,3, 1
1Biology Department, Indian Institute of Science Education and Research, 2Department of Biology, Center for Genomics and Systems Biology, New York University, 3Enveda Therapeutics India Pvt Ltd.

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El presente protocolo describe la creación de cultivos 3D "en la parte superior " de una línea celular epitelial de mama no transformada, MCF10A, que se ha modificado para estudiar la transformación inducida por el factor activador plaquetario (PAF). La inmunofluorescencia se ha utilizado para evaluar la transformación y se discute en detalle.

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Investigación del cáncer Número 185 Mediador fosfolípido células MCF10A cultivos tridimensionales transformación PAF
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