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Fosfolipidmediatörinducerad transformation i tredimensionella kulturer

Article DOI: 10.3791/64146-v • 08:02 min • July 27th, 2022
July 27th, 2022 •
1, 1,2, 1,3, 1
1Biology Department, Indian Institute of Science Education and Research, 2Department of Biology, Center for Genomics and Systems Biology, New York University, 3Enveda Therapeutics India Pvt Ltd.

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Detta protokoll beskriver upprättandet av 3D "på toppen " -kulturer av en icke-transformerad bröstepitelcelllinje, MCF10A, som har modifierats för att studera trombocytaktiveringsfaktor (PAF) inducerad transformation. Immunofluorescens har använts för att bedöma transformationen och diskuteras i detalj.

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Cancerforskning Utgåva 185 Fosfolipidmediator MCF10A-celler tredimensionella kulturer transformation PAF
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