Titolo Incapsulamento cella di goccioline

Published: October 01, 2007
doi:

Protocol

NIH3T3 cellule preparazione: Le cellule A. espulsione Trypsinize cellule, poi diluire 1:1 con mezzi di comunicazione cellulare, e il trasferimento da un pallone T75 ad un tubo da 15 ml Falcon Spin le cellule in una pallina da centrifugazione, aspirare il surnatante e lavare le cellule con DPBS Spin le cellule in una pallina di nuovo e aspirare il surnatante Risospendere le cellule in mezzi di comunicazione Determinare la densità delle cellule …

Disclosures

The authors have nothing to disclose.

Materials

Material Name Type Company Catalogue Number Comment
Fluorescent Microscope   Nikon Eclipse TE-2000 U  
Solenoid valve cell ejector       Operation frequency: 1 KHz, 30psi, 50nl~0.5ul. 12V Valve Driver: 2.5 Amp drive current
5-Gallon Portable air tank   Coleman Powermate CT5 Pressurized air: 30 PSI
Pressure regulators   Marsh Bellofram    
Pulse Generator     HP8112A Actuation frequency generation: 50 MHz
XYZ-stage   Newmark Systems   With Stepping motor: 6inch travel xy-stage(NLS4-6-25), 2.5inch travel z-stage(NLS4-2.5-25), 3axis controller(NSC-G3), 0.1um resolution
NIH 3T3 Cell-line     fibroblasts
Trypsin       0.05% solution
NIH 3T3 cell medium        
DPBS Buffer      
T75       Tissue culture flasks
Plastic conical tubes       15 ml, for tissue culture

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Cite This Article
Moon, S., Lin, P., Keles, H. O., Yoo, S., Demirci, U. Title Cell Encapsulation by Droplets. J. Vis. Exp. (8), e316, doi:10.3791/316 (2007).

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