This video and protocol demonstrate how to implant a glass-covered cranial window in rodents. These preparations can be used for chronic in vivo two-photon imaging of the neocortex over time scales of months. It may also be used for other types of imaging, including optical intrinsic signal imaging.
As we have shown in the video and in the supplementary figures, the cranial window preparation, combined with the use of two-photon microscopy, is a very powerful tool to study in vivo the structure and function of the neocortex. The technique requires rigorous training to become familiar with the relevant anatomy and the fine surgical procedures and skills that this preparation requires. Only pristine surgeries can be used for chronic imaging. If the dura is manipulated excessively or punctured, the preparation should not be used for imaging.
Material Name | Type | Company | Catalogue Number | Comment |
---|---|---|---|---|
Carprofen (Rimadyl) | Drug | Pfizer | ||
Isoflurane (Aerrane) | Surgery | Baxter | ||
Dexamethasone | Drug | Baxter | ||
Ortho-Jet Powder | Reagent | LANG | To be mixed with the acrylic | |
Jet-Acrylic Liquid | Reagent | LANG | To be mixed with Ortho-Jet Powder | |
Round Glass Cover Slip | Tool | Electron Microscopy Sciences | 72195-05 | 5 mm diameter |
Gelfoam | Surgery | Pharmacia & Upjohn Co. (Pfizer) |