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Biology

计数和确定培养细胞的可行性

doi: 10.3791/752 Published: June 23, 2008

Summary

重要的是,在培养条件的标准化和进行准确的定量实验确定文化中的细胞数量。在这段视频中,我们展示了如何使用一个hemacytometer细胞计数。

Abstract

重要的是,在培养条件的标准化和进行准确的定量实验确定文化中的细胞数量。一个hemacytometer是与中心区作为计数室设计了一个厚厚的玻璃幻灯片。适用于某个特定地区的细胞悬液,计数,这样可以计算出细胞密度。

Protocol

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这个实验方法的完整的文本协议是可在细胞生物学中的电流协议。

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计数和确定培养细胞的可行性
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Ricardo, R., Phelan, K. Counting and Determining the Viability of Cultured Cells. J. Vis. Exp. (16), e752, doi:10.3791/752 (2008).More

Ricardo, R., Phelan, K. Counting and Determining the Viability of Cultured Cells. J. Vis. Exp. (16), e752, doi:10.3791/752 (2008).

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