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Journal
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Biology
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焦距钙
2 +</sup瞬态检测平滑肌
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Focal Ca
2+
Transient Detection in Smooth Muscle
Please note that all translations are automatically generated.
Click here for the English version.
焦距钙
2 +</sup瞬态检测平滑肌
DOI:
10.3791/1247-v
•
17:41 min
•
June 29, 2009
•
John S. Young
,
Robert J. Amos
,
Keith L. Brain
1
Department of Pharmacology
,
University of Oxford
Chapters
00:05
Title
00:56
Introduction
01:30
Preparing the Ca2+ Indicator, Dissecting the Tissue, and Loading the Ca2+ Indicator
05:05
Preparing the ‘Ca2+ Loaded’ Tissue for Microscopy
08:44
Preparing for Analysis and Correcting for Movement
11:57
Particle Detection Algorithm
16:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
高分辨率CA的详细信息的方法<sup> 2 +</sup>成像在孤立的器官,包括平滑肌组织,图像采集和数据分析的准备。
Tags
Focal Ca2+ Transient Detection
Smooth Muscle
Ca2+ Imaging
Cellular Mechanisms
Membrane Potential
Internal Stores
Multiple Cells
Coupling
Subcellular Ca2+ Transients
Stochastic Occurrence
Wide Field Of View
Contraction
Imaging Protocols
Analysis Routines
Frequency
Location
Amplitude
Purinergic Ca2+ Transients
Transmitter Release
Submicron Resolution
ATP Release
P2X1 Receptors
Detrusor
Vas Deferens
Purinergic Neuroeffector Ca2+ Transients (NCTs)
Optical Monitoring
Neurotransmitter Release
Spatial Resolution
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