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Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

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Biology

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JoVE Journal Biology

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

DOI:

10.3791/63157-v

•

05:50 min

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November 01, 2021

•

Nikola Lukic*¹, Trishna Saha*¹, Stefanie Lapetina, Michal Gendler, Gilad Lehmann, Anthony J. Koleske³, Hava Gil-Henn

¹The Azrieli Faculty of Medicine,Bar-Ilan University, ²Department of Molecular Biophysics and Biochemistry,Yale University, ³Department of Neuroscience,Yale University

Chapters

00:04Introduction
00:47Glass-Bottom Dish Coating
01:48Preparation of Cells for Imaging
02:27Microscope Setup and Imaging
03:23Image Analysis
04:29Results: Cell-Edge Protrusion Assay at the Edge of Spreading Cells
05:08Conclusion

Summary

Automatic Translation

This protocol aims to measure the dynamic parameters (protrusions, retractions, ruffles) of protrusions at the edge of spreading cells.

Tags

Keywords: Cell-edge Protrusion Cell Migration Live-cell Microscopy Cell Motility Fibronectin BSA Trypsin Hemacytometer Phase Contrast Microscopy

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