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Journal
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Immunology and Infection
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特定细胞群的净化荧光激活细胞分选(FACS)
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
Please note that all translations are automatically generated.
Click here for the English version.
特定细胞群的净化荧光激活细胞分选(FACS)
DOI:
10.3791/1546-v
•
15:29 min
•
July 10, 2010
•
Sreemanti Basu
,
Hope M. Campbell
,
Bonnie N. Dittel
,
Avijit Ray
1
Blood Research Institute
,
BloodCenter of Wisconsin
Chapters
00:05
Title
00:08
Introduction
00:38
Materials and Preparation for FACS
02:17
Cell Staining & Prep of Control Samples
07:11
Flow Cytometer Setup
08:56
Flow Cytometer Compensation
11:58
Experimental Sample Gating & Sorting
14:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
许多科学研究需要的细胞群的生物和化学分析,细胞必须在高纯度的状态。荧光激活细胞分选(FACS)是一种高级的方法,在其中获得纯的细胞群。
Tags
Purification
Specific Cell Population
Fluorescence Activated Cell Sorting
FACS
Technique
Phenotype
Bulk Methods
Panning
Complement Depletion
Magnetic Bead Separation
Advantages
High Purity
Identifying Marker
Differential Marker Density
Internal Staining
Intracellular Protein Expression
Genetically Modified Fluorescent Protein Marker
Size
Granularity
Fluorescence
Fluorescently-tagged Monoclonal Antibodies
Negative Selection
Flow Cytometer
Sorting Capacity
Software
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