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Journal
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Immunology and Infection
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蛍光活性化細胞選別(FACS)によって特定の細胞集団の精製
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
Please note that all translations are automatically generated.
Click here for the English version.
蛍光活性化細胞選別(FACS)によって特定の細胞集団の精製
DOI:
10.3791/1546-v
•
15:29 min
•
July 10, 2010
•
Sreemanti Basu
,
Hope M. Campbell
,
Bonnie N. Dittel
,
Avijit Ray
1
Blood Research Institute
,
BloodCenter of Wisconsin
Chapters
00:05
Title
00:08
Introduction
00:38
Materials and Preparation for FACS
02:17
Cell Staining & Prep of Control Samples
07:11
Flow Cytometer Setup
08:56
Flow Cytometer Compensation
11:58
Experimental Sample Gating & Sorting
14:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
細胞集団の生物学的、化学的分析を必要とする多くの科学的研究のために細胞は純度の高い状態になっている必要があります。蛍光活性化細胞選別(FACS)は、純粋な細胞集団を取得するための優れた方法です。
Tags
Purification
Specific Cell Population
Fluorescence Activated Cell Sorting
FACS
Technique
Phenotype
Bulk Methods
Panning
Complement Depletion
Magnetic Bead Separation
Advantages
High Purity
Identifying Marker
Differential Marker Density
Internal Staining
Intracellular Protein Expression
Genetically Modified Fluorescent Protein Marker
Size
Granularity
Fluorescence
Fluorescently-tagged Monoclonal Antibodies
Negative Selection
Flow Cytometer
Sorting Capacity
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