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Biology

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Mätning Plasma membranprotein Endocytic priser genom reversibel Biotinylation
 
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Mätning Plasma membranprotein Endocytic priser genom reversibel Biotinylation

Article DOI: 10.3791/1669
December 23rd, 2009

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Summary December 23rd, 2009

Please note that all translations are automatically generated.

Click here for the English version.

Reglerad endocytos styr cellens nivåerna ytan uttryck för majoriteten av membranproteiner. Här använder vi reducera, membran impermeant biotinylation reagenser för att mäta endocytic hastighet av dopamin transportören (DAT), en polytopic membranprotein. Metoden möjliggör en okomplicerad metod för att mäta endocytic andelen flesta plasma membranproteiner.

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