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Zuivering van het M. magneticum Strain AMB-1 Magnetosome geassocieerd eiwit MamAΔ41
JoVE Journal
Biology
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JoVE Journal Biology
Purification of the M. magneticum Strain AMB-1 Magnetosome Associated Protein MamAΔ41
DOI:

11:07 min

March 25, 2010

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Chapters

  • 00:00Title
  • 00:42Introduction
  • 01:10MamA∆41 Protein Expression
  • 02:21MamA∆ 41 Proteing Extraction
  • 05:26Fast Performance Liquid Chromatography (FPLC)
  • 07:57MamA∆41 Protein Purity Evaluation
  • 08:50Representative Results of MamA∆41 Protein Purification
  • 10:40Conclusion

Summary

Automatic Translation

Mama is een uniek Magnetosome geassocieerd eiwit dat werd aangetoond dat het betrokken is bij magnetosome activering. Hier presenteren we de zuivering protocol van Mama deletiemutant (MamAΔ41) van<em> M. magneticum</em> AMB-1.

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