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一个型号的不安流致动脉粥样硬化小鼠颈动脉部分结扎术和颈动脉内皮细胞的RNA分离的简单方法
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JoVE Journal Medicine
A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium
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一个型号的不安流致动脉粥样硬化小鼠颈动脉部分结扎术和颈动脉内皮细胞的RNA分离的简单方法

DOI:
10.3791/1861-v

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11:00 min

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June 22, 2010

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, , , , , , , ,
1Department of Medicine, Division of Cardiology,Emory University, 2Coulter Department of Biomedical Engineering,Georgia Tech and Emory University, 3Department of Bioinspired Science,Ewha Womans University

Chapters

  • 00:00Title
  • 00:42Introduction
  • 01:24Partial Ligation of the Left Carotid Artery
  • 04:21Carotid Ultrasound Examination to Validate Disturbed Carotid Flow
  • 06:28Intimal RNA Isolation From Carotid
  • 09:19Representative Results
  • 10:11Conclusion

Summary

Automatic Translation

Automatic Translation

这描述了一个局部的颈动脉结扎手术，这会导致不安的水流条件和随后的动脉粥样硬化的发展（在两个星期）intraplaque新生血管在小鼠左颈总动脉（在四个星期内）。我们还描述了一个新颖的RNA从颈动脉内膜隔离方法，提供高纯度的内皮细胞的RNA。

Tags

Disturbed Flow-induced AtherosclerosisMouse Carotid ArteryPartial LigationRNA IsolationEndotheliumAtherogenesisLow Flow ConditionsOscillatory FlowCommon Carotid ArteryDirect EvidenceRapid Atherosclerosis DevelopmentApolipoprotein E Knockout MouseEndothelial DysfunctionHyperlipidemic Mouse ModelComplex Lesion FormationIntraplaque NeovascularizationMolecular MechanismsFlow-dependent RegulationVascular BiologyTherapeutic Interventions

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