The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow

Zaman Mirzadeh; Fiona Doetsch; Kazunobu Sawamoto; Hynek Wichterle; Arturo Alvarez-Buylla

doi:10.3791/1938

Journal

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Neuroscience

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The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow

41,609 Views

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14:33 min

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May 06, 2010

DOI:

10.3791/1938-v

DOI:

10.3791/1938-v

•

14:33 min

May 06, 2010

•

22 Views

Zaman Mirzadeh¹, Fiona Doetsch^2,3, Kazunobu Sawamoto⁴, Hynek Wichterle^2,5, Arturo Alvarez-Buylla¹

¹Department of Neurosurgery, The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California, San Francisco – UCSF, ²Department of Pathology and Cell Biology,College of Physicians and Surgeons, Columbia University, ³Department of Neuroscience and Neurology,College of Physicians and Surgeons, Columbia University, ⁴Department of Developmental and Regenerative Biology,Nagoya City University Graduate School of Medical Sciences, ⁵Center for Motor Neuron Biology and Disease,College of Physicians and Surgeons, Columbia University

Summary

The lateral ventricle walls contain the largest germinal region in the adult mammalian brain. Traditionally, studies on neurogenesis in this region have relied on classical sectioning techniques for histological analysis. Here we present an alternative approach, the wholemount technique, which provides a comprehensive, en-face view of this germinal region.