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花香浸改造拟南芥检查 pTSO2：β-葡萄糖醛酸酶报告基因的表达
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Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
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花香浸改造拟南芥检查 pTSO2：β-葡萄糖醛酸酶报告基因的表达

DOI:
10.3791/1952-v

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10:24 min

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June 11, 2010

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, ,
1Department of Cell Biology and Molecular Genetics,University of Maryland College Park

Chapters

  • 00:00Title
  • 01:00Introduction
  • 01:35Floral-dip Transformation of Arabidopsis thaliana
  • 05:20Selecting for Transgenic Plants
  • 07:25Examining pTS02::GUS Expression Patterns
  • 09:26Representative Images of GUS Expression in Arabidopsis thaliana Transformants
  • 09:57Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了花卉浸方法<em>农杆菌</em>介导的转化<em>拟南芥</em>。通过引入一个细胞周期调节的发起人，记者，<em> pTSO2：β-葡萄糖醛酸酶（GUS）的</em>，到<em>拟南芥</em>，我们演示了如何检测到GUS基因在转基因苗记者表达。

Tags

Floral-dip TransformationArabidopsis ThalianaPTSO2::β-glucuronidase Reporter Gene ExpressionForeign GenesModern BiologyBiotechnologyAgrobacterium TumefaciensPlasmid ConstructsArabidopsis ResearchPlant Gene FunctionReporter Geneβ-glucuronidase (GUS)TSO2 PromoterRibonucleotide Reductase (RNR)DNDP BiosynthesisS-phase Of The Cell CycleTransgenic Arabidopsis SeedlingsEducation

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