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Floral-dip Transformation von Arabidopsis thaliana Zum Untersuchen PTSO2: β-Glucuronidase Reporter Gene Expression
JoVE Journal
Biology
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JoVE Journal Biology
Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
DOI:

10:24 min

June 11, 2010

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Chapters

  • 00:00Title
  • 01:00Introduction
  • 01:35Floral-dip Transformation of Arabidopsis thaliana
  • 05:20Selecting for Transgenic Plants
  • 07:25Examining pTS02::GUS Expression Patterns
  • 09:26Representative Images of GUS Expression in Arabidopsis thaliana Transformants
  • 09:57Conclusion

Summary

Automatic Translation

Dieser Artikel veranschaulicht die floral-dip-Methode der<em> Agrobacterium tumefaciens</em>-Vermittelte Transformation von<em> Arabidopsis thaliana</em>. Durch die Einführung eines Zellzyklus-regulierten Promotor-Reporter<em> PTSO2: β-Glucuronidase (GUS)</em>, In<em> Arabidopsis</em>, Zeigt uns, wie man GUS-Reporter Expression in transgenen Keimlinge erkennt.

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