A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 01:00Introduction
- 01:35Floral-dip Transformation of Arabidopsis thaliana
- 05:20Selecting for Transgenic Plants
- 07:25Examining pTS02::GUS Expression Patterns
- 09:26Representative Images of GUS Expression in Arabidopsis thaliana Transformants
- 09:57Conclusion
En este artículo se ilustra el método de inmersión floral de<em> Agrobacterium tumefaciens</em> La transformación mediada de<em> Arabidopsis thaliana</em>. Mediante la introducción de un ciclo celular regulados promotor-reportero,<em> PTSO2:: β-glucuronidasa (GUS)</em>, En<em> Arabidopsis</em>, Que ilustra cómo se detecta la expresión de GUS en plantas transgénicas reportero.
Tags
Floral-dip TransformationArabidopsis ThalianaPTSO2::β-glucuronidase Reporter Gene ExpressionForeign GenesModern BiologyBiotechnologyAgrobacterium TumefaciensPlasmid ConstructsArabidopsis ResearchPlant Gene FunctionReporter Geneβ-glucuronidase (GUS)TSO2 PromoterRibonucleotide Reductase (RNR)DNDP BiosynthesisS-phase Of The Cell CycleTransgenic Arabidopsis SeedlingsEducation
