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Examination of the Telomere G-overhang Structure in Trypanosoma brucei
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Chapters
- 00:05Title
- 01:12Introduction
- 01:59Detection of T. brucei Telomere G-overhang Using Native In-Gel Hybridization
- 07:18Determination of the Telomere Nucleotide by Adaptor Ligation
- 09:48Ligation Mediated Primer Extension
- 11:46Representative Results for the Examination of the Telomere G-overhang Structure
- 15:00Conclusion
Telomeres are essential for chromosome stability and the telomere G-overhang structure is essential for telomerase-mediated telomere maintenance. We have recently adopted two methods for detecting the telomere G-overhang structure in Trypanosoma brucei, which are native in-gel hybridization and ligation-mediated primer extension, which will be described.
