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Mätning Calpain aktivitet inom fast och levande celler med flödescytometri
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
DOI:

11:37 min

July 08, 2010

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Chapters

  • 00:06Title
  • 00:48Introduction
  • 01:15Reagent Preparation
  • 01:56Cell Preparation
  • 03:11Calpain Assay in Fixed Cells
  • 04:48Data Acquisition (Fixed Cells)
  • 06:55Calpain Assay in Live Cells
  • 08:22Calpain Assay in Mixed Cells
  • 10:05Analysis and Representative Results
  • 11:09Conclusion

Summary

Automatic Translation

Denna artikel kommer detaljerat protokoll för mätning calpain aktivitet i fasta och levande celler med hjälp av flödescytometri.

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