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Electroporation of Plasmid DNA into Mouse Skeletal Muscle

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Biology

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JoVE Journal Biology

Electroporation of Plasmid DNA into Mouse Skeletal Muscle

Electroporation of Plasmid DNA into Mouse Skeletal Muscle

DOI:

10.3791/63916-v

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06:20 min

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April 06, 2022

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Brian A. Hain, David L. Waning

¹Department of Cellular and Molecular Physiology,Penn State College of Medicine

Chapters

00:04Introduction
00:55Tibialis Anterior (TA) Muscle Injection/Electroporation
02:55EDL Injection/Electroporation
04:35Results: Transient Gene Expression Modulation in Murine Skeletal Muscle by Plasmid Electroporation
05:31Conclusion

Summary

Automatic Translation

Electroporation of plasmid DNA into skeletal muscle is a viable method to modulate gene expression without compromising muscle contractility in mice.

Tags

Keywords: Electroporation Plasmid DNA Mouse Skeletal Muscle Gene Transfer Muscle Contractility In Vivo Tibialis Anterior Needle Injection Caliper Electrodes Square Wave Pulses

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