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成人和胚胎骨骼的肌肉Microexplant文化和骨骼肌肉干细胞的分离
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
成人和胚胎骨骼的肌肉Microexplant文化和骨骼肌肉干细胞的分离
DOI:
10.3791/2051-v
•
14:36 min
•
September 21, 2010
•
Deborah Merrick
,
Hung-Chih Chen
,
Dean Larner
,
Janet Smith
1
School of Biosciences
,
University of Birmingham
Chapters
00:00
Title
01:51
Introduction
02:38
Establishing Primary Skeletal Muscle Microexplant Cultures
05:33
Establishing Primary Microexplant Cultures from Embryos
09:43
in vitro
Analysis of Skeletal Muscle Stem Cells and Primary Cultures
11:15
Representative Results of Skeletal Muscle Microexplant Culture
14:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
微解剖植技术是一个强大而可靠的隔离,从少年,成年或胚胎骨骼肌干细胞的来源肌肉增生骨骼肌细胞的方法。与众不同的是,这些细胞已被克隆派生的骨骼肌干细胞体内移植使用的线。
Tags
Skeletal Muscle Microexplant Culture
Isolation Of Skeletal Muscle Stem Cells
Embryonic Skeletal Muscle Cells
Adult Skeletal Muscle Cells
Micro-dissected Explants Technique
Proliferative Skeletal Muscle Cells
Growth Characteristics
Cell Survival
Proliferation
Senescence
Differentiation
Explant Outgrowth Rates
Primary Cultures
Embryonic Skeletal Muscle Precursors
Clonal SMSc Lines
In Vivo Transplantation
Satellite Cells
Skeletal Muscle Fibre Regeneration
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